TNF alpha anticorps

N° du produit ABIN1176946

Cet anticorps Souris Monoclonal détecte spécifiquement TNF alpha dans ELISA et Neut. Il présente une réactivité envers Humain et a été mentionné dans 2+ publications.

Aperçu rapide pour TNF alpha anticorps (ABIN1176946)

Antigène: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp TNF alpha est non-conjugé

Application: ELISA, Neutralization (Neut)

Clone: MAb1

Détail du produit anti-TNF alpha anticorps

Marque: BD Pharmingen™

Attributs du produit: Neutralization Activity: This antibody has been reported to be useful for the neutralization of recombinant human TNF. Neutralization activity may be measured using a cytolysis assay for MTT dye conversion with 2.0 ng/mL recombinant human TNF (Cat. No. 554618) and L929 at 2x10^5 cells/mL as indicator cells (i.e. preincubation of the antibody with recombinant human TNF for 60 minutes).
50% Neutralization (ND50) at 20 - 1200 ng/mL
> 85% Neutralization at 2 - 15 µg/mL

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Stérilité: 0.2 μm filtered

niveau d'endotoxine: Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.

Immunogène: Human TNF Recombinant Protein

Isotype: IgG1

Information d'application

Indications d'application: ELISA: The purified format of the MAb1 antibody (Cat. No. 551220) is useful as a capture antibody for a sandwich ELISA for measuring human TNF. The purified MAb1 antibody can be paired with the biotinylated MAb11 antibody (Cat. No. 554511) as the detection antibody, with recombinant human TNF protein (Cat. No. 554618) as the standard. For complex biological samples, such as plasma or serum, investigators are encouraged to consider utilizing the BD OptEIA™ ELISA Set (Cat. No. 555212) or BD OptEIA™ ELISA Kit (Cat. No. 550610).

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.

Agent conservateur: Azide free

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

Références pour anticorps anti-TNF alpha (ABIN1176946)

Rathjen, Cowan, Furphy, Aston: "Antigenic structure of human tumour necrosis factor: recognition of distinct regions of TNF alpha by different tumour cell receptors." dans: Molecular immunology, Vol. 28, Issue 1-2, pp. 79-86, (1991) (PubMed).

Hogan, Vogel: "Production of tumor necrosis factor by rIFN-gamma-primed C3H/HeJ (Lpsd) macrophages requires the presence of lipid A-associated proteins." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 141, Issue 12, pp. 4196-202, (1989) (PubMed).

Détails sur TNF alpha

Antigène: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Autre désignation: TNF

Sujet: The MAb1 antibody reacts with human tumor necrosis factor (TNF, aka TNF-alpha) protein. TNF is a multifunctional cytokine involved in a variety of immune and inflammatory responses including hemorrhagic necrosis of tumors, septic shock, fever and autoimmune diseases. TNF can regulate the growth and differentiation of many different cell types. It is produced by different activated cell types including macrophages, T lymphocytes, NK cells, dendritic cells, endothelial cells, peripheral blood leukocytes, osteoblasts, astrocytes, mast cells, Kupffer cells, smooth muscle cells, fibroblasts and certain tumor cells. TNF exists in two biologically active forms, i.e., transmembrane and soluble forms. Upon activation, cells express transmembrane TNF glycoproteins that associate as homotrimeric complexes. After enzymatic cleavage, the extracellular region of membrane TNF sheds as a soluble homotrimer. The biologically active form of TNF has been reported to be a trimer.

Pathways: Signalisation NF-kappaB, Apoptose, Caspase Cascade in Apoptosis, Signalisation TLR, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome